Forestry and Environment Sympoisum 1998, Sri Lanka

Fourth Annual Symposium organized by Department of Forestry and Environment Science, University of Sri Jayewardenapura, Sri Lanka.

Wednesday, October 04, 2006

EFFECT OF THE PERIOD OF THE YEAR WHICH EXPLANTS WERE COLLECTED ON SHOOT GROWTH AND THE CALLUS FORMATION OF THE TISSUE CULTURED JACK FRUIT

K A H K Kasthuri Arachchi and W T P S K Senarath
Department of Botany, University of Sri Jayewardenepura,
Nugegoda.


A method for rapid propagation of mature Jack fruit from apical meristem culture was developed. Apical meristems were established in Modified Campbell and Durzan medium supplemented with NAA and IBA. Cultures were sub cultured in every 4 weeks interval in order to reduce the accumulation of phenolic compounds. Reducing the accumulated phenolics at the base of the explant enhanced the growth rate. There was a significant difference in the growth performance of shoots and callus produced according to the period of the year in which explants were collected.

60% of the apical meristems cultured in modified CD medium supplemented with IBA and NAA produced shoots in November to December period. It was only 30% when the apices were cultured in April to May months and decreased to 20% in June - July months. The shoots produced in November - December period showed a higher vigour (in number of leaves per shoot, mean leaf width and mean shoot length) than those produced in other months. Since jackfruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been effected by endogenous rhythms. It has been observed that the callus production depends on the incubation temperature. Callus was induced at the base of the shoot by increasing the incubation temperature from 25±1"C to 30±1°C. Growth of the callus was also retarded by accumulated phenolic compounds in the medium.

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